tnf-a試劑盒影響因素

試驗(yàn)與測(cè)定方法

細(xì)胞因子主要是根據(jù)其生物學(xué)活性和免疫學(xué)檢測(cè)，生物試驗(yàn)是確定生物活性和免疫學(xué)檢測(cè)是測(cè)定蛋白質(zhì)的重量或體積濃度。這兩種信息對(duì)于了解細(xì)胞因子在疾病中的作用是非常重要的。由于總蛋白量的細(xì)胞因子和免疫根據(jù)檢測(cè)的生物活性的測(cè)定可能會(huì)有所不同，所以在觀察時(shí)應(yīng)注意不同的方法不能比較。

2。細(xì)胞因子免疫學(xué)檢測(cè)方法主要采用酶聯(lián)免疫吸附法或放射免疫法。不同試劑盒對(duì)樣品有不同的要求，標(biāo)本按照試劑盒說(shuō)明書的要求保存

[influencing factors]
Test and determination method of 1. cytokines is mainly based on their biological activity and immunological tests, biological tests is to determine the biological activity and immunological test is the determination of the weight or volume of protein concentration. These two kinds of information are important for understanding the role of cytokines in disease. Due to the total protein amount of cytokines and immune according to the inspection by the determination of biological activity may be different, so in the clinical observation should pay attention to different methods can not be compared.

[意義] TNF主要由巨噬細(xì)胞和單核細(xì)胞產(chǎn)生。人們已經(jīng)發(fā)現(xiàn)，TNF-α可以產(chǎn)生多器官的生物學(xué)效應(yīng)，更重要的是在substance.tnf功能在細(xì)胞因子網(wǎng)絡(luò)具有多種生物學(xué)效應(yīng)，主要是通過(guò)抗腫瘤和免疫調(diào)節(jié)功能的免疫介導(dǎo)的，也是炎癥反應(yīng)的介質(zhì)，在炎癥性病變的病理變化許多方面。血清或其他體液中的TNF濃度，是基礎(chǔ)醫(yī)學(xué)和疾病診斷和治療價(jià)值的指標(biāo)。

1。腫瘤：許多腫瘤細(xì)胞可以產(chǎn)生腫瘤壞死因子，因此在許多腫瘤時(shí)，腫瘤壞死因子表達(dá)明顯增加，腫瘤壞死因子可以殺死腫瘤細(xì)胞，或通過(guò)cytotoxicity

2抑制某些腫瘤細(xì)胞的增殖。類風(fēng)濕關(guān)節(jié)炎患者有大量TNF。在他們的synovium3。在膿毒癥、肺炎等嚴(yán)重炎癥性疾病時(shí)血清TNF含量顯著升高。腫瘤壞死性寄生蟲病患者明顯改變。TNF患者體液免疫患者高于正常人。瘧疾抗原，病毒和細(xì)菌可以誘導(dǎo)腫瘤壞死因子TNF生物試劑，反過(guò)來(lái)，抗病毒，抗細(xì)菌，抗瘧疾role.4.tnf是移植排斥反應(yīng)密切相關(guān)，與腫瘤壞死因子的表達(dá)與血清或尿液排斥程度呈正相關(guān)
 

2. the immunological test method of cytokines mainly uses ELISA or RIA. Different reagent kits have different requirements for the samples, and the specimens are kept in accordance with the requirements of the kit instructions
[clinical significance]
TNF is mainly produced by macrophages and monocytes. It has been found that TNF can produce biological effects on many organs, it is more important in the function of substance.TNF in the cytokine network has a variety of biological effects, is mainly mediated by the immune function of anti-tumor and immune regulation, also is one of the mediators of inflammatory responses, many aspects of pathology the changes in inflammatory lesions. Serum or other body fluids in the concentration of TNF, is the basic medicine and clinical diseases diagnosis and treatment value of the index.
1. tumor: many tumor cells can produce TNF, so in many tumor time, TNF expression is obviously increased,.TNF can kill tumor cells or inhibit the proliferation of some tumor cells through cytotoxicity
2. rheumatoid arthritis patients have large amounts of TNF. in their synovium
3. in sepsis, pneumonia and other serious inflammatory diseases when the content of TNF in serum was significantly increased. Many patients with parasitic diseases in TNF was significantly changed. The humoral AIDS patients in TNF was higher than that of normal people. Malarial antigen, virus and bacteria can induce the production of TNF, TNF in turn, anti-virus, anti bacteria, anti malaria role.
4.TNF is closely related to graft rejection, and the expression of TNF is positively correlated with the extent of rejection in serum or urine